摘要

BACKGROUND:Endovascular embolization with liquid materials has been identified as a useful therapy for aneurysms. The materials with little toxicity,good biocompatibility,and the ability to induce effective cellular response within the vessels are ideal in the treatment of aneurysms. OBJECTIVE:To investigate the feasibility of RADA16-Ⅰ(Ac-RADARADARADA RADA-NH2),a nanofiber scaffold material as an embolic agent,in a rat aneurysm model. DESIGN,TIME AND SETTING:Randomized,controlled,animal experiment. The study was performed at Science Park,West China Hospital,Sichuan University between March and September 2008. MATERIALS:RADA16-Ⅰ powder was synthesized by Shanghai Bootech Bioscience Technology Co.,Ltd.; cellulose acetate polymer (CAP) was purchased from Sinopharm Chemical Reagent Co.,Ltd. METHODS:①Experiment in vitro:The frequency sweep rheological test of 10 g/L RADA16-Ⅰwith or without the phosphate buffer solution (PBS) was performed. ②Animal surgery:15 female Wistar rats were randomly divided into 3 groups:10 g/L RADA16-Ⅰgroup,CAP group and sham surgery group with 5 animals in each group. Under anesthesia,the right carotid was carefully exposed,RADA16-Ⅰor CAP were injected into the common carotid artery (CCA) to induce carotid aneurysm. The sham surgery group only underwent arterial ligation but not embolism treatment. MAIN OUTCOME MEASURES:①TA Instruments Advantage Software was used to analyze the rheological properties of 10 g/L RADA16-Ⅰwith or without PBS. ②The embolized CCAs were harvested 14 days after surgery,followed by HE staining and Masson staining. α smooth muscle actin,the marker in the vascular smooth muscle,in 10 g/L RADA16-Ⅰgroup was immunohistochemically examined. RESULTS:①10 g/L RADA16-Ⅰwith PBS possessed the capability of elastic behavior,similar to standard. ② According to HE and Masson staining,the vessel walls thickened apparently in the sham surgery group. The vessel walls of the CAP group become thinner and there was pink (HE) or blue (Masson) material in the vessels. In the 10 g/L RADA16-Ⅰgroup,the vessel walls become thickened,and the neointima cells grew into lumina; moreover,the material was degraded. According to immunohistochemical examination,the majority of neointima cells were vascular smooth muscle cells. CONCLUSION:RADA16-Ⅰis a potential embolic agent.

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